Chemical tests for alkaloids ppt

Tannins: Classification, Properties and Chemical Tests

Synonym is Loban, Sumatra Benzoin. Styrax benzoin is the biological source of benzoin and contains resin. These trees are not grown in India. It has a greyish-brown colour, aromatic odour, sweet taste. Cinnamic acid, benzoic acid, summaresinollic acid are the important chemical constituents.

Aim: Aim of the experiment is to carry out different chemical test to analyze the crude drug of benzoin. Conical flask.

Drug sample. Weight balance. Sulphuric acid. Ferric chloride solution. Chemical test. The above experiment shows the presence of chemical constituent in drug sample.

It is used as antiseptic, for the preparation of cosmetics, expectorant, carminative, stimulant and diuretics. Using stage micrometer calibrate the eyepiece micrometer. Calculate the factor average distance between two lines in microns. Stomatal number is defined as the average number of stomata per sq mm of epidermis of the leaf. Calibrate eyepiece micrometer by using stage micrometer and calculate the factor.

Mount a little quantity of powdered sample in These are the dried flower buds of Eugenia caryophyllus belonging to the family Myrtaceae. The present study is aimed Moisture content determination is important, not only to know excess water, but also in conjunction with suitable temperature moisture The chromatography column is made with plastic tip with frit, the Lycopodium spores are obtained from club moss, Lycopodium clavatum Linn.

The spores are yellow in We Labmonk, some scientific researchers unite to design a platform for getting sources of different lab protocols and discuss various research related issues. All rights reserved. Toggle navigation. Home Different chemical test to analyze the crude drug of benzoin.

chemical tests for alkaloids ppt

Different chemical test to analyze the crude drug of benzoin. Due to the presence of cinnamic acid a bitter almond like odour is produced. This test shows negative result for Siam benzoin. In a china dish, drops of sulphuric acid is added to the 0. Reddish-brown colour confirms the presence of Sumatra benzoin and purple-red colour confirms the presence of Siam benzoin.

Ferric chloride solution is added to alcoholic solution of benzoin. Due to the presence of phenolic compound coniferyl benzoate in Siam benzoin, it will produce green colour.

This test gives a negative result for Sumatra benzoin as it does not contain phenolic compund.In this article we will discuss about the tests for the detection of alkaloids in plants with the help of experiment. The alkaloids are a group of basic, secondary plant substance, which usually possess a N-containing hetero-cycle.

Their basic character is due to this feature. The alkaloids are mostly colourless, crystalline and non-volatile solids. They are insoluble in water, but soluble in organic solvents viz. The plant material is dried and finally powdered.

The extraction is made with ethanol by solvent distillation process. Then the extract is purified by various methods. However, for qualitative detection, crude extract can be used for chemical tests. Then dil.

H 2 SO 4 is added to the mixture. White or yellow precipitate is formed. Top Menu BiologyDiscussion. Estimation of Acetylcholine Esterase Enzyme Activity. Study of Community Structure Plant Ecology.

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Answer Now and help others. Answer Now. Here's how it works: Anybody can ask a question Anybody can answer The best answers are voted up and rise to the top.Abdel Nasser Singab. Why we study alkaloids? Importance of alkaloids as medicine? The poppy plant, Papaver somniferumis the source for non-synthetic narcotics. Tropane alkaloids antispasmodic etc Taxol the best anti-ovarian cancer CNS stimulant caffeine, ephedrine etc Identification of alkaloids, chemical test specific. Alkaloids are a group of mole cules distributed everywhere.

Alkaloids represent a group of natural produc ts that had a great influence, all over history on the economic, medical, political and social affairs of humans. Millions of people around the Globe use purine alkaloids every day whether starting the day with a cup of coffee or drinking a cup of tea in the afternoon.

Many have potent physiological effects and therefore, are considered as important therapeutic agents e. They are widely used to treat diseases ranging from malaria to cancer. Alkaloids are biomolecules of secondary metabolites which are derived from amino acids or from the transamination process and are classified according to the amino acids that provide their nitrogen atom and part of their skeleton. Similar alkaloids have different biosynthetic pathways. Alkaloids are derived from l-lysine, l- ornithine, l-tyrosine, l-tryptophane, l-histidine, l-phenylalanine, nicotinic acid, anthranilic acid or acetate.

chemical tests for alkaloids ppt

Alkaloids also occur in the animal ki ngdom. Alkaloids mean alkali-like substancesare basic nitrogenous compounds of plant or animal origin and generally possessing a marked physiological action on man or animals.

All alkaloids are nitrogenous, but not all the nitrogenous compounds are alkaloids. Plants are a rich source of alkaloids but some have been found in animals e. Ergot alkaloids and almost all alkaloids have been synthesized.

Most but not all possess basic properties due to the presence of an amino nitrogen but certain are amphoteric e. A Plant sources. B Animal sources. Recently alkaloids were found in animals and insects e. Ergot alkaloids; ergotamine and ergometrine from Ergot fungus.

Lycopodine from Lycopodium spores, and muscopyridine from the Musk deer. In general, alkaloids occur in a salt form with organic or inorganic acids, or in combination with specific acids e. Opium alkaloids occur with meconic acid and Cinchona alkaloids with cinchotannic acid. Some occur in combination with sugars as glycosides e. They play the following functions in plants:.Copy embed code:. Automatically changes to Flash or non-Flash embed. WordPress Embed Customize Embed.

chemical tests for alkaloids ppt

URL: Copy. Presentation Description This ppt includes almost complete info about alkaloids in general. The first civilizations to use them were probably the ancient Sumerians and Egyptians it was called a vegetable alkali. Term alkaloid was coined by meissner ,a German pharmacist Derosne French chemist isolated Narcotine Grecian times considered as an extremely powerful aphrodisiac and fertility enhancer.

Mentioned in the Koranand strictly forbidden. The sponge that was given to Jesus on the cross is said to contain Mandragora juice. Witches prepared mixtures of extracts of henbane Hyoscyamus n i gerdeadly nightshade Atropa belladonna and mandrake root, to be applied on the skin or on the genitals.

Analgesic action :- Morphine oral;mgi. Anti-cancer activity. Cytrabine Pyrimidine antagonists is an Anti-cancer drug 1. Vinblastine mitotic inhibitor is an anti-cancer drug 0. Oxalic, acetic acids Salts with inorganic acids e.

The alkaloids and Dragendorff's reagent

Hcl, H2SO4. Salts with special acids : e. Meconic acid in Opium, Quinic acid in Cinchona Glycosidal form e. Ranunculaceae : Aconitine aconite. Legumioceae : physostigmine physostigma. Papavaraceae : Morphine, Codiene, Thebaine Opium. Solanaceae : hyoscine Belladona. PowerPoint Presentation: f. Rubiaceae: Quinine and Quinidine Cinchona.

Liliaceae : Veriterine Veratrum.

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Clavicipitaceae Ergotamine and Ergometrine Ergot. Pyocyanine from Pseudomonas aeruginosa a bacteria. Muscopyridine From Musk of an Deer. Most alkaloids are crystalline solidbut some are liquid e. Volatile ; - -NicotineConiine.Tannins are naturally occurring complex organic compounds possessing nitrogen free polyphenols of high molecular weight.

They form colloidal solution with water giving acid reactions. They also precipitate proteins and alkaloids. The astringent in nature of tannins is due to the fact that they can precipitate proteins and render them resistant to enzymatic attack. When applied on a wound or injury, tannins form a protective coating so as to prevent external irritation and thus promote healing. These tannins are hydrolyzed by acids, or enzyme and produce gallic acid and ellagic acid. Chemically, these are esters of phenolic acid like gallic acid and ellagic acid.

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The tannins derived from gallic acid are known as gallitannins and from that of ellagic acid are known as gallitannins. The gallic acid is found in rhubarb, clove and ellagic acid is found in eucalyptus leave and myrobalans and pomegranate bark. These tannins treated with ferric chloride to produced blue or black colour. These tannins are resistant to hydrolysis and they derived from the flavonols, catechins and flavan-3, 4-diols.

On treatment with acids or enzymes they are decomposed into phlobaphenes. On dry distillation condensed tannin produce catechol. These tannins are called as catechol tannins. These tannins are found in cinchona bark, male fern, areca seeds, tea leaves and wild cherry bark, bahera fruits, Amla, etc.

PPT. Alkaloids

They are found in catechu and nux- vomica, etc. Tannins are freely soluble in water, alcohol, glycerol, and acetone and dilute alkalies. They are sparingly soluble in chloroform, ethyl acetate and other organic solvents.

They have an astringent taste. They yield purple, violet or black precipitate with iron compounds. They are precipitated by number of metallic salts notably potassium dichromate, and lead acetate and sub acetate. They combine with skin and hide to form leather and with gelatin and isinglass to form an insoluble compound.

They combine with alkaloids to form tannates, most of which are insoluble in water. To a solution of tannin, aqueous solution of gelatin and sodium chloride are added. A white buff coloured precipitate is formed. The skin piece is washed with distilled water and kept in a solution of ferrous sulphate.Biotech Articles.

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Publish Your Research Online. Article Summary: It is a semi quantitative method to find out the presence of sugars that is polysaccharides. We have to hydrolyze the drug and then chemical tests are carried out. The procedure for the isolation of glycosides also varies.

The general method is Stass Otto method Different tests are done because of the different chemical entities of the aglycone moiety. The general method is Stass Otto method. Anthracene glycosides 2. Cardiac glycosides 3. Saponin glycosides 4. Flavonoidal glycosides 5.

Cyanogenetic glycoside 6. Isothiocyanate glycoside 7. Phenol glycoside 8. Alcoholic glycoside 9. Lactone glycoside Bitter glycoside Aldehydic glycoside. Classification according to the linkage between aglycone and glycone.

O-glycoside: e. S-glycoside: Isothiocyanate glycoside that is sinigrin from black mustard 4. N- glycoside: they are present in nucleosides where amino group of base reacts with -OH group of ribose or deoxy ribose to form n-glycoside.

They are also classified according to the type of sugars present: e. Glucoside which contain glucose Rhamnoside which contain Rhamnose Pentoside which contain pentose sugars etc. Which includes glucose or rhamnose along with deoxy sugar like digitoxose.

At the junction of the liquids reddish-brown colour is produced which gradually becomes blue. Baljet tests, Legal tests, Raymond tests for the presence of lactone ring. Important Disclaimer: All articles on this website are for general information only and is not a professional or experts advice.

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Please see our disclaimer section for complete terms. Agriculture Bioinformatics Applications Biotech Products. Industry News Issues Nanotechnology Others.Alkaloids are a class of naturally occurring organic compounds that mostly contain basic nitrogen atoms.

This group also includes some related compounds with neutral [2] and even weakly acidic properties. Alkaloids are produced by a large variety of organisms including bacteriafungiplantsand animals.

Other alkaloids possess psychotropic e. Alkaloids can be toxic too e. The boundary between alkaloids and other nitrogen-containing natural compounds is not clear-cut. There is no unique method for naming alkaloids. There are also at least 86 alkaloids whose names contain the root "vin" because they are extracted from vinca plants such as Vinca rosea Catharanthus roseus ; [25] these are called vinca alkaloids. Alkaloid-containing plants have been used by humans since ancient times for therapeutic and recreational purposes.

For example, medicinal plants have been known in Mesopotamia from about BC. It is believed that the gift was an opium-containing drug. Extracts from plants containing toxic alkaloids, such as aconitine and tubocurarinewere used since antiquity for poisoning arrows. Studies of alkaloids began in the 19th century. Several other alkaloids were discovered around that time, including xanthineatropinecaffeineconiinenicotinecolchicinesparteineand cocaine The first complete synthesis of an alkaloid was achieved in by the German chemist Albert Ladenburg.

He produced coniine by reacting 2-methylpyridine with acetaldehyde and reducing the resulting 2-propenyl pyridine with sodium. Compared with most other classes of natural compounds, alkaloids are characterized by a great structural diversity. There is no uniform classification. This classification is now considered obsolete.

More recent classifications are based on similarity of the carbon skeleton e. Alkaloids are often divided into the following major groups: [41]. Some alkaloids do not have the carbon skeleton characteristic of their group. So, galanthamine and homoaporphines do not contain isoquinoline fragment, but are, in general, attributed to isoquinoline alkaloids.

Most alkaloids contain oxygen in their molecular structure; those compounds are usually colorless crystals at ambient conditions. Oxygen-free alkaloids, such as nicotine [] or coniine[35] are typically volatile, colorless, oily liquids. Most alkaloids are weak bases, but some, such as theobromine and theophyllineare amphoteric.

Alkaloids and acids form salts of various strengths. These salts are usually freely soluble in water and ethanol and poorly soluble in most organic solvents. Exceptions include scopolamine hydrobromide, which is soluble in organic solvents, and the water-soluble quinine sulfate. Most alkaloids have a bitter taste or are poisonous when ingested.

Alkaloid production in plants appeared to have evolved in response to feeding by herbivorous animals; however, some animals have evolved the ability to detoxify alkaloids.

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